Enhancements    

MMUNOPURIFICATION USING MONOCLONAL ANTIBODIES TO MOJAVE TOXIN

MMUNOPURIFICATION USING MONOCLONAL ANTIBODIES TO MOJAVE TOXIN
Inventors: Ebbie Rael, Richard Salo
Patent No. 5,053,492

Description:
Monoclonal antibodies to Mojave toxin and use for isolation of cross-reacting proteins in Crotalus venoms are disclosed. Hybridomas secreting monoclonal antibodies against Mojave toxin were established. The antibodies were used for identifying cross-reacting proteins in individual C. s. scutulatus and other Crotalus venoms and to isolate Mojave toxin. The antibodies recognized five bands with a pI range from 5.1 to 6.1 in immunoblots of electrofocused crude venom and Mojave toxin purified by immunoaffinity chromatography. The specificity of the antibodies was for the basic subunit of the toxin which resolved into four bands of pI between 9.3 and 9.6. Individual C. s. scutulatus venoms of snakes from Texas and southern Arizona had multiple bands with pI's ranging from 4.9 to 6.3. Cross-reacting proteins were also recognized by antibodies in the electrophoresed venoms of C. basiliscus, C. d. durissus, C. d. terrificus, C. h. horridus, and C. v. concolor, and may be isolated by immunoaffinity chromatography with the monoclonal antibodies.

Abstract:
The present invention is directed to monoclonal antibodies to Mojave toxin which are useful in the isolation of Mojave toxin and immunologically related toxins found in the Crotalus genus of snakes.

Issue Date: 10/01/1991
Application Date: 08/07/1986
Post Date: 07/26/2019

UTEP Docket No: 1986-001