Magnetic Separation of Cells

Magnetic Separation of Cells
Inventors: Thomas Boland, Sylvia Natividad, Julio Rincon
Patent No. 9,034,589

This patent works by combining inkjet printing technology and magnetic labeling of cells. By doing this, accurate cell counts for efficient disease diagnosis are obtained using an optical microscope and imaging software.

An apparatus, system, and method for magnetic separation of cells are disclosed. By combining inkjet printing technology and magnetic labeling of cells, accurate cell counts are obtained using an optical microscope. Mouse CD4+ lymphocytes are attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer. The labeled cells are then attached to a glass slide covering a permanent magnet. Cell counts can be obtained by use of regular and inverted optical microscopes and imaging software. The magnetically-labeled beads are collected for evaluation on a modified polymer coupon that is placed in front of a permanent magnet and the unlabeled cells fall into an excess container. Flow cytometry results verify the presence of the CD4+ protein on the LBRM-33 lymphocytes membrane. Protein-specific attachment of magnetic microspheres to the lymphocytes is utilized for sorting CD4+ lymphocytes.

Issue Date: 05/19/2015
Application Date: 03/22/2013
Post Date: 03/02/2018

UTEP Docket No: 2012-001